High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer
1 BioSilta Oy, P. O. Box 4300, FI-90014 Oulu, Finland
2 Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering, University of Oulu, P. O. Box 4300, FI-90014 Oulu, Finland
3 Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, Ackerstrasse 71-76, D-13355 Berlin, Germany
Microbial Cell Factories 2011, 10:107 doi:10.1186/1475-2859-10-107Published: 12 December 2011
This report describes the combined use of an enzyme-based glucose release system (EnBase®) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli. Compared to Terrific Broth and ZYM-5052 autoinduction medium, the EnBase system improved yield mainly through increased productivity per cell. Four-fold increase in oxygen transfer by the Ultra Yield Flask contributed to higher cell density with EnBase but not with the other tested media, and consequently the product yield per ml of EnBase culture was further improved.