Table 2

Primer and hydrolysis probe sequences for qPCR, amplicon length and PCR efficiencies

Gene

Primer 1 (5' - 3')

Primer 2 (5' - 3')

Amplicon length

Hydrolysis probe

Efficiency cDNA

Efficiency plasmid

crtW148

ttgtgcccaaacaattagcc

ttcctcgtggtagccaaaat

73

#119

1.88

1.83

crtZ

cgatctttatgccgtggttt

ggccacattcctgtactgc

72

#18

1.95

1.91

hpd

ctatctgatcgaccgcttcg

ccttcgatgaagttgaagtcg

66

#8

1.94

2

Lemuth et al. Microbial Cell Factories 2011 10:29   doi:10.1186/1475-2859-10-29