Table 1

Genetic engineering strategies which have been applied to the enhancement of recombinant protein production in bacteria

Method

Strain

Target protein

Reference

Spontaneous chromosomal mutagenesis

C(1(DE3), C43(DE3)

Bovine OGCP; bovine phosphate carrier; bovine ADP/ATP translocase; Bacillus subtilis PS3 alanine/H⁺ carrier; E. coli F-ATPase subunits b and c; bovine F-ATPase subunits b, d, α, β, γ, δ, OSCP, F₆; bovine F-ATPase inhibitor protein; Aequoria victoria GFP

[58]

Chromosomal mutagenesis using chemical mutagens or mutator genes

EXP-Rv1337-1, EXP-Rv1337-2, EXP-Rv1337-3, EXP-Rv1337-4, EXP-Rv1337-5

Mycobacterium tuberculosis Rv1337, M. tuberculosis Rv2746, M. tuberculosis Rv2835, M. tuberculosis Rv0110, Methanocccus jannaschii rhomboid (MJR), Drosophila melanogaster rhomboid 1 (Rho1)

[60]

GS1, TM1, TM2, TM3, TM4, TM5, TM6

Variants of human IgG1

[61]

Transposon mutagenesis

GS101 (MC4100A dnaJ349::Tn5 (Kan^R))

GS102 (MC4100A dinG1377::Tn5 (Kan^R))

GS103 (MC4100A nhaR63::Tn5 (Kan^R))

GS104 (MC4100A ΔdinG)

GS105 (MC4100A ΔdinG dnaJ349::Tn5 (Kan^R))

Human central cannabinoid receptor (CB1)

[62]

Co-expression of the ASKA library

MC4100A (+ybaB)

MC4100A (+yciQ)

MC4100A (+glpQ)*

Human bradykinin receptor 2 (BR2), CB1, human neurokinin (substance P) receptor 1 (NKR1), E. coli YidC, E. coli CstA, human stearoyl-CoA desaturase (SCD)

[64]

*MC 100A (+name of gene) denotes an MC 100 strain overexpressing the E. coli gene specified in parentheses.

Makino et al. Microbial Cell Factories 2011 10:32   doi:10.1186/1475-2859-10-32