Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure
1 University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, 1190 Vienna, Austria
2 University of Applied Sciences FH-Campus Vienna, School of Bioengineering, Muthgasse 18, 1190 Vienna, Austria
3 University of Natural Resources and Life Sciences, Department of Chemistry, Muthgasse 18, 1190 Vienna, Austria
4 Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 11, 1190 Vienna, Austria
Microbial Cell Factories 2011, 10:47 doi:10.1186/1475-2859-10-47Published: 26 June 2011
Additional file 1:
MATLAB implementation of the kinetic model. Time courses of the intracellular and extracellular labeled Fab concentrations after start of labeling are modeled dependent of the kinetic parameters of protein formation, degradation and secretion. Continuous cultivation (constant YDM and dilution rate) are prerequisits for numerically solving the differential equations.
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Additional file 2:
Sensitivity analysis of the kinetic parameters. At a time two of the parameters, namely degradation, secretion and intracellular protein formation are varied against each other to determine the effect on extracellular Fab3H6 concentrations. Three dimensional plots are automatically created using the MATLAB functions meshgrid and surf.
Format: M Size: 5KB Download file