Table 1

Effect of metal ions on the stability of alkaline α-amylase

Metal ions

Relative activity (%)b


1 mM

10 mM


CKa

100

100

Na+

110 ± 2

96 ± 2

Co2+

97 ± 2

96 ± 2

Zn2+

80 ± 1

85 ± 1

Ca2+

102 ± 2

85 ± 3

K+

97 ± 2

100 ± 2

Mn2+

37 ± 1

27 ± 3

Mg2+

86 ± 2

84 ± 2

Fe2+

74 ± 2

84 ± 2

Cu2+

81 ± 1

88 ± 1

Fe3+

90 ± 2

86 ± 1


a The relative activity was calculated on the basis of the activity (CK) obtained in glycine/NaOH buffer (pH 10.0) without the addition of any metal ions.

b Values represent means ± SD (n = 3) relative to the untreated control samples.

Yang et al. Microbial Cell Factories 2011 10:77   doi:10.1186/1475-2859-10-77

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