Table 1

Methylglyoxal synthase assay results.

mgsA source

Specific Activity (U/mg)

Km (mM)

Specific Activity/Km (U/mg/mM)


C. acetobutylicum

0.054 ± 0.004

0.776 ± 0.005

0.069

B. subtilis

0.056 ± 0.003

0.473 ± 0.07

0.118

C. difficile

0.059 ± 0.0.003

1.439 ± 0.06

0.041

E. coli

0.124 ± 0.006

1.418 ± 0.12

0.087

T. thermophilus

0.016 ± 0.004

2.118 ± 0.07

0.007

K. pneumoniae

0.016 ± 0.009

2.820 ± 0.3

0.005

P. fluorescens

0.013 ± 0.008

1.560 ± 0.02

0.008

R. eutropha

0.005 ± 0.000

0.700 ± 0.03

0.007


Substrate dihydroxyacetone phosphate concentration was varied from 0.15 mM to 1.5 mM for all reactions. 1 unit (U) was defined as the amount (μmoles) of methylglyoxal formed per unit time (min).

Jain and Yan Microbial Cell Factories 2011 10:97   doi:10.1186/1475-2859-10-97

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