Table 4

List of strains and plasmids used in this study.

Strain

Genotype

Reference


E. coli BW25113

rrnBT14 DlacZWJ16 hsdR514 DaraBADAH33 DrhaBADLD78

[32]

E. coli BL21*

F- ompT hsdSB (rB- mB-) gal dcm (DE3)

Invitrogen

E. coli XL-1 Blue

recA1 endA1gyrA96thi-1hsdR17supE44relA1lac [F' proAB lacIqZDM15Tn10 (TetR)]

Stratagene


Plasmid

Description

Reference


pZE12-luc

pLlacO1::luc(VF); ColE1 ori; AmpR

[29]

pCS27

pLlacO1:: MCS; p15A ori; KanR

[7]

pCDF-Duet1

pT7lac::MCS;CDF ori; SmR

EMD Chemicals Inc., NJ

pYY93

ppdABC from K. oxytoca cloned into pCS27

This study

pYY109

budC from K. pneumoniae cloned into pCDF-Duet1

This study

pYY134

gldABC from K. pneumoniae cloned into pCS27

This study

pYY167

dhab12 from C. butyricum cloned into pCS27

This study

pRJ1

mgsA from C. acetobutylicum cloned into pZE12-luc

This study

pRJ2

mgsA from B. subtilis cloned into pZE12-luc

This study

pRJ3

mgsA from C. difficile cloned into pZE12-luc

This study

pRJ4

mgsA from E. coli cloned into pZE12-luc

This study

pRJ5

mgsA from T. thermophilus cloned into pZE12-luc

This study

pRJ6

mgsA from K. pneumoniae cloned into pZE12-luc

This study

pRJ7

mgsA from P. fluorescens cloned into pZE12-luc

This study

pRJ8

mgsA from R. eutropha cloned into pZE12-luc

This study

pRJ9

gldA from E. coli cloned into pZE12-luc

This study

pRJ10

ydjG from E. coli cloned into pZE12-luc

This study

pRJ11

ydjG from E. coli, budC from K. pneumoniae, and mgsA

from B. subtilis cloned in pZE12-luc

This study


Jain and Yan Microbial Cell Factories 2011 10:97   doi:10.1186/1475-2859-10-97

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