Recent contributions in the field of the recombinant expression of disulfide bonded proteins in bacteria
Department of Environmental Sciences, University of Nova Gorica, Vipavska 13, Rozna Dolina (Nova Gorica), PO Box301, SI-5000, Slovenia
Microbial Cell Factories 2012, 11:129 doi:10.1186/1475-2859-11-129Published: 14 September 2012
The production of heterologous disulfide bonded proteins in bacteria remains a biotechnological challenge. A rapid literature survey results in the identification of some interesting proposals, such as the option of producing functional proteins in the cytoplasm in the presence of sulfhydryl oxidases and isomerases. Furthermore, an ever-increasing number of applications refers to recombinant proteins displayed at the bacterial surface. Time will tell whether these developments will lead to universally accepted laboratory protocols.