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Open Access Research

Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Rocío Gómez-Pastor2, Roberto Pérez-Torrado2, Elisa Cabiscol3, Joaquim Ros3 and Emilia Matallana12*

Author Affiliations

1 Departamento de Bioquímica y Biología Molecular, Universitat de València, Valencia, Spain

2 Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Apartado de Correos, 73. Burjassot (Valencia). E-46100, Spain

3 Departament de Ciències Mèdiques Bàsiques, IRBLleida, Universitat de Lleida, Spain

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Microbial Cell Factories 2012, 11:4  doi:10.1186/1475-2859-11-4

Published: 9 January 2012

Abstract

Background

In the yeast biomass production process, protein carbonylation has severe adverse effects since it diminishes biomass yield and profitability of industrial production plants. However, this significant detriment of yeast performance can be alleviated by increasing thioredoxins levels. Thioredoxins are important antioxidant defenses implicated in many functions in cells, and their primordial functions include scavenging of reactive oxygen species that produce dramatic and irreversible alterations such as protein carbonylation.

Results

In this work we have found several proteins specifically protected by yeast Thioredoxin 2 (Trx2p). Bidimensional electrophoresis and carbonylated protein identification from TRX-deficient and TRX-overexpressing cells revealed that glycolysis and fermentation-related proteins are specific targets of Trx2p protection. Indeed, the TRX2 overexpressing strain presented increased activity of the central carbon metabolism enzymes. Interestingly, Trx2p specifically preserved alcohol dehydrogenase I (Adh1p) from carbonylation, decreased oligomer aggregates and increased its enzymatic activity.

Conclusions

The identified proteins suggest that the fermentative capacity detriment observed under industrial conditions in T73 wine commercial strain results from the oxidative carbonylation of specific glycolytic and fermentation enzymes. Indeed, increased thioredoxin levels enhance the performance of key fermentation enzymes such as Adh1p, which consequently increases fermentative capacity.

Keywords:
Thioredoxins; Carbonylation; Yeasts; Biomass; Stress