A generalised module for the selective extracellular accumulation of recombinant proteins
1 School of Immunity and Infection, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
2 School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
3 School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom
Microbial Cell Factories 2012, 11:69 doi:10.1186/1475-2859-11-69Published: 28 May 2012
It is widely believed that laboratory strains of Escherichia coli, including those used for industrial production of proteins, do not secrete proteins to the extracellular milieu.
Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems.
The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications.