Schematic processes of genome-scale metabolic model reconstruction for K. oxytoca. (1-2-3) Reconstruction of metabolic network using genome annotation data. (3-6-7-2 or 4-5-7-2) Manual curation and model refinement of metabolic network based on literatures, databases, gene-protein-reaction correlation, comparative genome analysis, error corrections, and gap filling. (8-9-10) Model validation by comparing with experimental data and determination of biomass composition to be applied to the model. (11–12) Strain improvement and metabolic engineering by combining with in silico simulation and analysis. The thick and black arrows indicate the main process for model reconstruction. The thin and gray arrows indicated the process of model refinement and improvement.
Park et al. Microbial Cell Factories 2013 12:20 doi:10.1186/1475-2859-12-20