Effects of varying oxygen uptake rate for 2,3-BD production. (A) In silico prediction to examine the response of 2,3-BD production rate to varying oxygen uptake rate from 0 to 30 mmol/gDCW/h. (B) Fermentation profiles for different agitation speeds (150, 250, 350, 450, 550, 650, and 750 rpms) and DO levels (5% and 10%) in ldhA gene knockout mutant of K. oxytoca. The open circle (○), filled circle (●), and filled square (■) indicate the concentrations of glucose, 2,3-BD, and OD600, respectively. (C) Summary of fermentation results that include ending time of fermentation (hour), final DCW (g/L), 2,3-BD concentration (g/L), yield (g/g glucose), and productivity (g/L/h).
Park et al. Microbial Cell Factories 2013 12:20 doi:10.1186/1475-2859-12-20