A: Characteristics of a substrate limited fed-batch technique with constant temperature. B: Characteristics of a temperature limited fed-batch technique in which the substrate concentration is controlled at a non-limiting concentration while DOT is controlled at a set-point by means of the temperature controller. Parameters in A and B correspond to growth of P. pastoris on glycerol which promotes cell accumulation only. After a certain cell density is reached, the production phase can be started by a shift to methanol feed. F: feed rate; X: biomass concentration; My (μ): specific growth rate; T: temperature; DOT: dissolved oxygen tension; S: substrate concentration.
Jahic et al. Microbial Cell Factories 2003 2:6 doi:10.1186/1475-2859-2-6