Figure 1.

A: Characteristics of a substrate limited fed-batch technique with constant temperature. B: Characteristics of a temperature limited fed-batch technique in which the substrate concentration is controlled at a non-limiting concentration while DOT is controlled at a set-point by means of the temperature controller. Parameters in A and B correspond to growth of P. pastoris on glycerol which promotes cell accumulation only. After a certain cell density is reached, the production phase can be started by a shift to methanol feed. F: feed rate; X: biomass concentration; My (μ): specific growth rate; T: temperature; DOT: dissolved oxygen tension; S: substrate concentration.

Jahic et al. Microbial Cell Factories 2003 2:6   doi:10.1186/1475-2859-2-6
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