Table 2

Membrane functional changes as a function of growth rate and cultivation technique

FUNCTION2

Continuous cultivation

Fed-batch cultivation

Comment


Parameter

Low μ

Min/Max

High μ

Low μ

Min/Max

High μ


Total protein production (per CDW)

R

High

High

R

High

High

Yield drops from the theoretical 0.5 to approx. 0.4

Specific protein production (per tot prod.)

R

High

High.

R

High

High

Model substance: periplasmic β-lactamase

Cell lysis (%)

8

1

1

1

<1

<1

Effect of sonication (% release of specific protein)

56

78

73

57

80

85

Result of 1 min sonication

Endotoxin release (% of CDW)

1

1

1

<1

<1

<1

Periplasmic leakage

1.5

19

7

10

10

9


2Summary of the structural differences in continuous and fed-batch cultivation as a function of growth rate. Results compared to data from Shokri et al 2002. A high growth rate refers to μ = 0.6 h-1 and a low growth rate to μ = 0.05 h-1.

Shokri and Larsson Microbial Cell Factories 2004 3:9   doi:10.1186/1475-2859-3-9

Open Data