Table 1

Overview of various applications of the NICE system

Application area
Application
Reference

Expression of homologous genes
Aminopeptidase N
[7]

ATPase system (8 gene operon)
[28]

Aminoacylase
[29]

Cluster of genes encoding folate biosynthesis
[19]
Expression of heterologous genes of Gram+ and Gram- bacteria
NADH oxidase of Streptococcus mutans
[30]

Fructose bisphosphatase of Escherichia coli
[31]

Green fluorescent protein of Aequoria victoria
[32]

Lysostaphin of Staphylococcus simulans biovar. Staphylolyticus
[15]
Protein secretion
Lipase of Staphylococcus hyicus
[33]

Bovine beta-lactoglobulin
[34]
Membrane proteins: prokaryotic and eukaryotic
Multidrug transporter of Lactococcus lactis


Xylidose transporter of Lactobacillus pentosus


ATP/ADP translocator of Rickettsia prowazekii
Review [35]

KDEL receptor of Homo sapiens


Mitochondrial carriers of Saccharomyces cerevisiae

Cloning of toxic genes
Lysis cassette of the virulent phage us3 of
[26]

Lactococcus lactis


Autolysin gene of Leuconostoc citreum
[36]
Bacterial antigens
Antigen L7/12 of Brucella abortus
[13, 37]

C subunit of tetanus toxin (TTFC) of Clostridium tetani
[38]
Viral antigens
Non-structural protein 4 of bovine rotavirus
[13, 39]
Cytokines
Interleukin 12 of Homo sapiens
[40]
Industrial-scale application
Lysostaphin of Staphylococcus simulans biovar. Staphylolyticus
[15]