Figure 2.

Dependence of the expression level of the shorter analogue of UBP1, UBP1D2QL, on the time after IPTG induction as revealed by SDS electrophoresis in 12% polyacrylamide gel. E. coli BL21 (DE3) cells transformed by the plasmid pT7UPD2QL were cultured at 25°C in LB, and induced by the addition of IPTG to the final concentration of 1 mM. Aliquots of cultures were taken at: 1 – 0 min, 2 – 30 min, 3 – 60 min, 4 – 90 min, 5 – 120 min, 6 – 150 min after induction.