|
Primers for the construction of pT7RS and UBP1 derivatives |
||
| Primersa |
Sequencesb |
Restriction site |
|
|
||
| HIST7G |
5' AATTCGATATCGTCGACGGATCCCATCATCACCATCACCATTAAAAT 3' |
EcoRI |
| HIST7D |
5' AGCTATTTTAATGGTGATGGTGATGATGGGATCCGTCGACGATATCG 3' |
BamHI, SalI, EcoRV, |
| 6HisG |
5' TATGGCACATCATCACCATCACCACTCTGGTTCTG 3' |
NdeI |
| 6HisD |
5' AATTCAGAACCAGAGTGGTGATGGTGATGATGTGCCA 3' |
EcoRI |
| UB1G |
5' GGGGAATTCATATGCAGATTTTCGTCAAAACTTTG 3' |
EcoRI and NdeI |
| UBID2 |
5' GGGGATCCTTAATGCTCTTCACCACCGCGGAGTCTTAAG 3' |
BamHI and SacII |
| UBP1G |
5' AGACTCCGCGGTGGTGATTTGTTTATTGAAAGCAAGATA 3' |
SacII |
| UBP1D |
5' GGGGATCCTTAGTTTACATCTTTACCAGAAATA 3' |
BamHI |
| UBP1MG |
5'GGCATAGTAGTATTTTTTTACCGCGGTGGTGACCATCTAAACTACATTGT 3' |
SacII |
| UBP1MD |
5'ACAATGTAGTTTAGATGGTCACCACCGCGGTAAAAAAATACTACTATGCC 3' |
SacII |
| SkrutG |
5'AAAACCGCGGTGGTTTCATTGCTGGTTTA 3' |
SacII |
| SkrutD |
5'GGAAGAATTCTTGCGCGTCCTC 3' |
EcoRI |
| UBP1GC |
5'-GATTTGGAAGCTATTCAGAGTAATAATGAAG-3' |
|
| UBP1DC |
5'-CTTCATTATTACTCTGAATAGCTTCCAAATC-3' |
|
| KalaG |
5'-GATCCAGGCGATAAAGACGGTGATGGCTATATTTCTGCTGCTGAAGCTATGGCTTA-3' |
BamHI |
| KalaD |
5'-AGCTTAAGCCATAGCTTCAGCAGCAGAAATATAGCCATCACCGTCTTTATCGCCTG-3' |
HindIII |
|
a The names of primers contain the letters: G – sense or D – antisense. b The restriction sites are underlined or overlined. The histidine and leucine codons are in bold face. | ||
Wojtowicz et al. Microbial Cell Factories 2005 4:17 doi:10.1186/1475-2859-4-17 |
||