|
Plasmids and genes used to construct vectors of the pT7RS derivatives |
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| Vectors |
Gene cloned |
Result ing vectors |
Expression products |
Name of the protein used in the text |
|
|
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| pT7SR |
Ubiquitin |
pT7U |
Ubiquitin |
UBI |
| pT7U |
K |
pT7UK |
UBI::K |
UBI::K |
| UBP1 |
pT7UP |
UBI::UBP1 |
UBP1 |
|
| pT7CH |
UBI::UBP1 with Q754L mutation |
pT7UPQL |
UBI::UBPQL::6xHis |
UBPQL |
| UBI::UBP1(55-809) without Q754L mutation |
pT7UPD |
UBI::UBPD::6xHis |
UBPD |
|
| UBI::UBP1(55-809) with Q754L mutation |
pT7UPDQL |
UBI::UBPDQL::6xHis |
UBPDQL |
|
| pT7CH |
UBI::UBP1(99-809) with Q754L mutation |
pT7UPD2QL |
UBI::UBPD2QL::6xHis |
UBPD2QL |
| pT7NH |
Ubiquitin |
pT7NHU |
6xHis::UBI |
6xHisUBI |
| pT7NHU |
S |
pT7NHUS |
6xHis::UBI::S |
6xHisUBI::S |
Wojtowicz et al. Microbial Cell Factories 2005 4:17 doi:10.1186/1475-2859-4-17 |
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