Figure 4.

Fluorescence microscopy of EGFP and mSbsC-EGFP expressing yeast transformants. Yeast cells expressing EGFP (A) or mSbsC-EGFP (C) were cultivated in selective minimal medium to exponential phase and analysed by fluorescence microscopy. Westernblot analysis of whole cell extract using anti-GFP antibodies reveal the presence of either EGFP (B) or mSbsC-EGFP (D). bar = 2 μm