|
13C labeling patterns of amino acids in cell hydrolysates of Saccharomyces cerevisiae cultivatedon [1-13C] glucose in chemostat under aerobic glucose-limited conditions at different growth rates. |
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| Measured Metabolite |
Purely Oxidative Growth (μ = 0.15 h-1) |
Respiro-Fermentative Growth (μ = 0.30 h-1) |
Mainly Fermentative Growth (μ = 0.40 h-1) |
||||||
| M0 |
M1 |
M2 |
M0 |
M1 |
M2 |
M0 |
M1 |
M2 |
|
|
|
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| Alanine (m/z 260, C1–3) |
|||||||||
| calca |
0.647 |
0.328 |
0.596 |
0.380 |
0.539 |
0.435 |
|||
| expb |
0.652 |
0.331 |
0.597 |
0.381 |
0.543 |
0.442 |
|||
| Alanine (m/z 232, C2–3) |
|||||||||
| calc |
0.671 |
0.316 |
0.618 |
0.372 |
0.561 |
0.427 |
|||
| exp |
0.666 |
0.325 |
0.615 |
0.374 |
0.549 |
0.442 |
|||
| Glycine (m/z 246, C2) |
|||||||||
| calc |
0.922 |
0.074 |
0.932 |
0.065 |
0.964 |
0.035 |
|||
| exp |
0.922 |
0.074 |
0.931 |
0.064 |
0.963 |
0.036 |
|||
| Valine (m/z 186, C2–5) |
|||||||||
| calc |
0.450 |
0.424 |
0.117 |
0.365 |
0.457 |
0.165 |
0.308 |
0.471 |
0.203 |
| exp |
0.442 |
0.424 |
0.116 |
0.361 |
0.451 |
0.165 |
0.301 |
0.480 |
0.203 |
| Valine (m/z 260, C2–5) |
|||||||||
| calc |
0.450 |
0.424 |
0.117 |
0.365 |
0.457 |
0.165 |
0.308 |
0.471 |
0.203 |
| exp |
0.442 |
0.428 |
0.120 |
0.364 |
0.457 |
0.170 |
0.300 |
0.475 |
0.206 |
| Valine (m/z 288, C2–5) |
|||||||||
| calc |
0.434 |
0.425 |
0.128 |
0.346 |
0.451 |
0.179 |
0.288 |
0.464 |
0.220 |
| exp |
0.435 |
0.429 |
0.125 |
0.355 |
0.456 |
0.174 |
0.294 |
0.475 |
0.210 |
| Serine (m/z 288, C2–3) |
|||||||||
| calc |
0.694 |
0.303 |
0.628 |
0.369 |
0.566 |
0.430 |
|||
| exp |
0.693 |
0.300 |
0.624 |
0.366 |
0.569 |
0.422 |
|||
| Serine (m/z 362, C2–3) |
|||||||||
| calc |
0.694 |
0.303 |
0.628 |
0.369 |
0.566 |
0.430 |
|||
| exp |
0.693 |
0.302 |
0.623 |
0.369 |
0.567 |
0.424 |
|||
| Serine (m/z 390, C1–3) |
|||||||||
| calc |
0.682 |
0.311 |
0.611 |
0.375 |
0.560 |
0.431 |
|||
| exp |
0.682 |
0.308 |
0.617 |
0.370 |
0.564 |
0.428 |
|||
| Threonine (m/z 376, C2–4) |
|||||||||
| calc |
0.541 |
0.381 |
0.526 |
0.409 |
0.515 |
0.440 |
|||
| exp |
0.540 |
0.387 |
0.521 |
0.409 |
0.504 |
0.455 |
|||
| Threonine (m/z 404, C1–4) |
|||||||||
| calc |
0.496 |
0.396 |
0.504 |
0.413 |
0.488 |
0.444 |
|||
| exp |
0.500 |
0.398 |
0.505 |
0.411 |
0.496 |
0.455 |
|||
| Aspartate (m/z 418, C1–4) |
|||||||||
| calc |
0.496 |
0.396 |
0.504 |
0.413 |
0.488 |
0.444 |
|||
| exp |
0.502 |
0.399 |
0.506 |
0.415 |
0.499 |
0.454 |
|||
| Arginine (m/z 414, C1–5) |
|||||||||
| calc |
0.302 |
0.417 |
0.219 |
0.239 |
0.410 |
0.261 |
0.263 |
0.450 |
0.241 |
| exp |
0.309 |
0.415 |
0.218 |
0.245 |
0.406 |
0.260 |
0.263 |
0.450 |
0.244 |
| Phenylalanine (m/z 336, C1–9) |
|||||||||
| calc |
0.379 |
0.429 |
0.166 |
0.304 |
0.437 |
0.212 |
0.240 |
0.442 |
0.262 |
| exp |
0.371 |
0.417 |
0.166 |
0.305 |
0.431 |
0.212 |
0.238 |
0.437 |
0.263 |
| Phenylalanine (m/z 302, C1–2) |
|||||||||
| calc |
0.973 |
0.961 |
0.979 |
||||||
| exp |
0.957 |
0.952 |
0.975 |
||||||
| Tyrosine (m/z 466, C1–9) |
|||||||||
| calc |
0.379 |
0.429 |
0.166 |
0.304 |
0.437 |
0.212 |
0.240 |
0.442 |
0.262 |
| exp |
0.379 |
0.421 |
0.165 |
0.304 |
0.432 |
0.213 |
0.242 |
0.440 |
0.250 |
| Tyrosine (m/z 302, C1–2) |
|||||||||
| calc |
0.973 |
0.961 |
0.979 |
||||||
| exp |
0.972 |
0.964 |
0.966 |
||||||
| Glutamate (m/z 432, C1–5) |
|||||||||
| calc |
0.352 |
0.427 |
0.184 |
0.278 |
0.431 |
0.233 |
0.276 |
0.458 |
0.230 |
| exp |
0.353 |
0.429 |
0.184 |
0.278 |
0.432 |
0.233 |
0.279 |
0.467 |
0.228 |
|
a Calculated values predicted by the solution of the mathematical model corresponding to the optimized set of fluxes b Experimental values obtained by GC-MS analysis of TBDMS-derivatized amino acids | |||||||||
Frick and Wittmann Microbial Cell Factories 2005 4:30 doi:10.1186/1475-2859-4-30 |
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