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Overview of cell sorting applications in biotechnology |
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| General aim |
Sorting target |
Selected examples |
References |
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| Physiological research |
Viability, vitality |
bacteria, yeasts |
[2-8] |
| Protein engineering |
ligand binding |
antibody surface display |
[22-24] |
| peptide surface display |
[15, 16, 26-29] |
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| enzyme engineering |
intra- and extracellular enzymes |
[30-33] |
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| Cellular properties |
cell hybridization, cloning |
yeast hybridization, library cloning |
[37, 38] |
| promoter trapping |
bacteria |
[45, 46] |
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| robustness |
acid tolerance |
[51] |
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| process related properties |
high cell density, low growth rate |
[51-55] |
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| Overproduction |
product stained by immunofluorescence |
protein |
[1, 56-69] |
| Autofluorescence of product |
alkaloids |
[76] |
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| Unspecific staining |
FITC/antibiotic production |
[78] |
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Mattanovich and Borth Microbial Cell Factories 2006 5:12 doi:10.1186/1475-2859-5-12 |
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