|
Xylose and arabinose consumption rates and product yields from anaerobic batch fermentations by strains BWY02.XA (xylose and arabinose-growing laboratory strain), TMB 3400 (xylose-growing industrial strain) and TMB 3063 (xylose and arabinose-growing industrial strain). Defined mineral medium with mixtures of 20 g/l glucose and 20 g/l xylose or 20 g/l glucose and 20 g/l arabinose as well as 20 g/l glucose, 20 g/l xylose and 20 g/l arabinose were used as indicated. Product yields are calculated from the pentose phase of the fermentation. (n.a. not applicable; n.d. not detected) |
|||||||||
| Substrate |
Strain |
qxylose (g xylose h-1 g cells-1) |
qarabinose (g arabinose h-1 g cells-1) |
Yarabitol (g arabitol g arabinose-1) |
Yxylitol (g xylitol g xylose-1) |
Yglycerol (g glycerol g pentose-1) |
Yacetate (g acetate g pentose-1) |
Yethanol (g ethanol g pentose-1) |
final ethanol concentration (g l-1) |
|
|
|||||||||
| Glucose + xylose |
BWY02.XA |
0.1 ± 0.0 |
n.a. |
n.a. |
0.34 ± 0.01 |
0.12 ± 0.01 |
0.02 ± 0.00 |
0.23 ± 0.01 |
14.2 ± 0.7 |
| Glucose + arabinose |
BWY02.XA |
n.a. |
0.03 ± 0.00 |
0.77 ± 0.22 |
n.a. |
0.00 ± 0.02 |
0.10 ± 0.04 |
n.d. |
9.0 ± 2.0 |
| Glucose + xylose + arabinose |
BWY02.XA |
0.041 ± 0.080 |
0.035 ± 0.014 |
1.14 ± 0.18 |
0.33 ± 0.03 |
n.d. |
n.d. |
0.07 ± 0.1 |
10.4 ± 2.2 |
| TMB 3400 |
0.066 ± 0.015 |
0.01 ± 0.00 |
approx. 1* |
0.42 ± 0.00 |
0.01 ± 0.00 |
n.d. |
0.09 ± 0.0 |
12.8 ± 0.5 |
|
| TMB 3063 |
0.042 ± 0.002 |
0.029 ± 0.002 |
0.68 ± 0.17 |
0.11 ± 0.03 |
0.03 ± 0.00 |
0.06 ± 0.01 |
0.16 ± 0.03 |
14.7 ± 2.0 |
|
|
(* not possible to measure due to small amounts of arabinose consumed and arabitol produced) | |||||||||
Karhumaa et al. Microbial Cell Factories 2006 5:18 doi:10.1186/1475-2859-5-18 |
|||||||||