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Stoichiometric coefficients for batch fermentation of rifamycin B using Amycolatopsis maditerranei S699 |
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| Stoichiometric coefficientsa (moles of substrate. C-mole of biomass-1) |
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| Amino acids |
Glucose and amino acids |
Glucose and ammonia |
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| Y1,1,k = 0.51 |
Y2,1,k = 0.13 |
Y3,2 = 0.35 |
| Y1,3 = 1.30 |
Y2,2 = 0.24 |
Y3,6 = 0.16 |
| Y1,6 = 0.85 |
Y2,3 = 1.10 |
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a Amycolatopsis mediterranei S699 was cultivated in two media compositions. (i) Containing mixture of amino acids at a concentraton of 3.25 mM each and (ii) medium (i) supplemented wih glucose (80 g.l-1). Both the media were supplemented with 11 gl-1 CaCO3, 1 gl-1 KH2PO4, 1 gl-1 MgSO4, 0.01 gl-1 FeSO4, 0.05 gl-1 ZnSO4 and 0.003 gl-1 COCl2. Samples were taken at regular intervals to estimate concentrations of amino acids, rifamycin B, biomass, residual ammonia and glucose. Online data such as vent CO2 and dissolved oxygen was obtained from exit gas analyzer. The offline and online data was further used to estimate stoichiometric coefficients on respective substrate assimilation modes. Y i,j are stoichiometric coefficients where i is reaction number (refer equations in materials and methods) and j is the substrate for example : Amino acid (1) where k represents 20 different amino acids, glucose (2), CO2 (3), O2 (4), H2O (5), Ammonia (6). | ||
Bapat et al. Microbial Cell Factories 2006 5:32 doi:10.1186/1475-2859-5-32 |
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