Table 2

Model parameters for rifamycin B " fermentation using the strain Amycolatopsis mediterraneia
Model Parameteresb
Amino acids
 μ1max
 Ks1
 kNT1
 KI1
 μ2max
 Ks2
 KI2
 Ks2,2
 KI2,2
 kNT2
 Kp2
 KpI 2
 Kp2 2
 KpI2,2
 Math
Alanine
 0.0015
 0.0016
 0.0259
 0.0158
 0.0386
 2 × 10-6
 0.51
 0.000155
 0.0929
 0.5929
 1.12 × 10-5
 0.0112
 0.1124
 1.1236
 7.782 × 10-05
Glycine
 0.0241
 0.0004
 0.8507
 0.1551
 0.02
 0.003866
 0.1543
 0.003866
 0.1507
 0.1507
 1.33 × 10-5
 0.0133
 0.1333
 1.3333
 0.001899
Valine
 0.0001
 5.15 × 10-6
 0.5671
 0.4738
 0.02
 5.15 × 10-6
 0.4382
 5.15 × 10-6
 0.5671
 0.5671
 8.55 × 10-6
 0.0085
 0.0855
 0.8547
 8.963 × 10-06
Leucine
 0.0004
 9.5 × 10-6
 0.0387
 0.1583
 0.024
 1.56 × 10-5
 0.7835
 1.56 × 10-5
 0.4888
 0.0387
 7.63 × 10-6
 0.0076
 0.0763
 0.7634
 4.137 × 10-05
Isoleucine
 0.0057
 2.88 × 0-5
 0.0323
 0.0008
 0.0487
 2.88 × 10-5
 0.3545
 0.00082
 0.1783
 0.1783
 8.4 × 10-6
 0.0076
 0.0763
 0.7634
 0.0004403
Threonine
 0.0486
 0.0011
 0.1649
 0.0018
 0.0259
 0.002746
 0.5618
 0.00171
 0.1649
 0.1649
 9.52 × 10-6
 0.0084
 0.084
 0.8403
 0.0031509
Serine
 0.0191
 0.0187
 0.6791
 0.1634
 0.045
 0.018685
 0.936
 0.00018
 0.01
 0.6791
 8.7 × 10-6
 0.0095
 0.0952
 0.9524
 0.0014994
Proline
 0.0101
 0.0071
 0.1103
 0.092
 0.06
 3.0 × 10-4
 0.45
 0.00057
 0.9103
 0.9103
 7.52 × 10-6
 0.0087
 0.087
 0.8696
 4.724 × 10-06
Aspertic acid
 0.0106
 5.17 × 10-7
 0.0272
 0.9714
 0.052
 0.05171
 0.0271
 5.17 × 10-9
 0.1609
 0.1609
 6.71 × 10-6
 0.0075
 0.0752
 0.7519
 0.0085576
Methionine
 0.0091
 0.0007
 0.0476
 0.0404
 0.1
 7.42 × 10-5
 0.9429
 0.00142
 0.0007
 0.4758
 6.8 × 10-6
 0.0067
 0.0671
 0.6711
 6.855 × 10-05
Glutiamic acid
 0.0075
 0.0006
 0.4581
 0.1383
 0.075
 0.013622
 0.0283
 1.36 × 10-5
 0.3058
 0.3058
 6.06 × 10-6
 0.0068
 0.068
 0.6803
 0.0018939
Phenylalanine
 0.0042
 2.26 × 10-5
 0.0082
 0.045
 0.0602
 2.26 × 10-6
 0.925
 2.26 × 10-6
 0.0419
 0.0082
 6.84 × 10-6
 0.0061
 0.0606
 0.6061
 6.98 × 10-05
Glutamine
 0.0001
 3.59 × 10-5
 0.033
 0.0068
 3.51 × 10-5
 3.59 × 10-5
 0.0007
 3.59 × 10-5
 0.033
 0.033
 6.85 × 10-6
 0.0068
 0.0684
 0.6845
 5.523 × 10-07
Lysine
 0.0109
 1.62 × 10-7
 0.0636
 0.0581
 0.0798
 0.016222
 0.013
 0.00015
 0.8362
 0.8362
 6.45 × 10-6
 0.0068
 0.0685
 0.6849
 0.0040591
Histidine
 0.0157
 8.79 × 10-6
 0.0472
 0.0942
 0.0685
 8.79 × 10-9
 0.4009
 8.79 × 10-6
 0.7243
 0.7243
 5.52 × 10-6
 0.0065
 0.0645
 0.6452
 0.0036322
Tyrosine
 0.0116
 0.0001
 0.0567
 0.0151
 0.0722
 0.000151
 0.5095
 0.00015
 0.0367
 0.0367
 4.9 × 10-6
 0.0065
 0.0552
 0.5525
 0.0016529
Tryptophan
 0.3919
 1.49 × 10-5
 0.0241
 0.0049
 0.0392
 1.49 × 10-7
 0.4902
 1.49 × 10-5
 0.0241
 0.0241
 8.7 × 10-16
 0.0049
 0.049
 0.4902
 0.0100978

a The model parameters were obtained by fitting experimental data (batches shown in Figure 1 and 2) to the model using fmincon subroutine from Matlab.

b Unit of and are in h-1. Unit of rest of the parameters are in moles.L-1. The value of Math has been found to be 0.004 h-1 for all amino acids.

Bapat et al. Microbial Cell Factories 2006 5:32   doi:10.1186/1475-2859-5-32
Open Data