Figure 1.

Influence of calcium ions on PGA production and export in B. megaterium. MS941 carrying pRBBm23 (encoding SP_pga-PGA) was cultivated in LB medium with indicated concentrations of CaCl₂. Proteins from 1.5 mL cell-free growth medium were precipitated by ammonium sulfate, analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue G250. Lane M shows Precision Plus Protein Standard (Bio-Rad, Muenchen, Germany).