Figure 4.

Comparison of different leader peptides for the production and export of B. megaterium PGA. PGA was produced in shaking flask cultivation of B. megaterium MS941 and YYBm1 carrying either pRBBm23 (encoding SP_pga-PGA) or pRBBm49 (encoding SP_lipA-PGA) in LB medium containing tryptone from different companies. At OD_578nm of 0.4 pga expression was induced by the addition of 5 g L^-1 xylose to the growth medium. Samples were taken at various time points after induction. Proteins from 10 μL unconcentrated growth medium were separated by SDS-PAGE and stained with Coomassie Brilliant Blue G250. Biomass concentration and PGA volumetric activity 24 h after induction of recombinant gene expression are shown.