Open Access Research

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Michael Maurer1, Manfred Kühleitner2, Brigitte Gasser1 and Diethard Mattanovich1,3*

1 University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Institute of Applied Microbiology, Vienna, Austria

2 University of Natural Resources and Applied Life Sciences Vienna, Department of Integrative Biology, Institute of Mathematics, Vienna, Austria

3 School of Bioengineering, University of Applied Sciences FH-Campus Vienna, Austria

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Microbial Cell Factories 2006, 5:37 doi:10.1186/1475-2859-5-37

Published: 11 December 2006

Additional files

Additional File 1:

Model of P. pastoris fed batch process for secreted heterologous protein production. This file contains the model as described in Material and Methods, with the optimized values for μ and dt. By adjusting the specific parameters, other fed batch processes can be modeled and optimized with the Excel Solver.

Format: XLS Size: 102KB Download file

This file can be viewed with: Microsoft Excel Viewer

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