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This article is part of the supplement: The 4th Recombinant Protein Production Meeting: a comparative view on host physiology

Open Access Poster Presentation

Protein expression for structural studies

Yoav Peleg1,6*, Shira Albeck1,6, Yigal Burstein2,6, Orly Dym1,6, Yossi Jacobovitch1,6, Nurit Levy1,6, Ran Meged1,6, Yigal Michael1,6, Jaime Prilusky3,6, Gideon Schreiber4,6, Israel Silman5,6, Tamar Unger1,6 and Joel L Sussman1,6

  • * Corresponding author: Yoav Peleg

Author Affiliations

1 Department of Structural Biology, The Weizmann Institute of Science, Rehovot 76100, Israel

2 Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel

3 Department of Biological Services, The Weizmann Institute of Science, Rehovot 76100, Israel

4 Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel

5 Department of Neurobiology, The Weizmann Institute of Science, Rehovot 76100, Israel

6 the Israel Structural Proteomics Center (ISPC). The Weizmann Institute of Science, Rehovot 76100, Israel

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Microbial Cell Factories 2006, 5(Suppl 1):P40 doi:10.1186/1475-2859-5-S1-P40

Published: 10 October 2006

First paragraph (this article has no abstract)

Protein structure determination is an essential tool for studying protein function, and assists the design of novel drugs. The goal of the Israel Structural Proteomics Center (ISPC) is to determine the structures of proteins related to human health in their functional context http://www.weizmann.ac.il/ISPC webcite[1]. One of the bottlenecks encountered, primarily for eukaryotic proteins, is the production of soluble and correctly folded proteins suitable for crystallization trials. In order to overcome this obstacle, we have applied various expression strategies. E. coli is the expression system of choice, in which different parameters are tested in parallel. In cases in which post-translational modification is essential for obtaining a functional protein, eukaryotic expression systems such as Pichia pastoris or baculovirus are being employed.