Table 1

Experimental design of RSM studies using two independent variables with three centre points, showing observed and predicted values of vanillin molar yield and specific productivity.

Vanillin Molar yield (%)

Specific Productivity (μmol vanillin/g biomass × min)

Experiment

Biomass (g/L)

Ferulic acid (mM)

Observed

Predicted

Observed

Predicted


1

1.0 (-1)

0.5 (-1)

65.4

66.4

4.27

4.01

2

4.5 (0)

0.5 (-1)

75.3

74.9

1.61

1.90

3

8.0 (1)

0.5 (-1)

68.0

66.9

0.88

0.67

4

1.0 (-1)

2.8 (0)

54.4

52.6

6.92

7.33

5

4.5 (0)

2.8 (0)

68.4

68.3

5.94

5.80

6

8.0 (1)

2.8 (0)

65.0

67.4

4.57

4.79

7

1.0 (-1)

5.0 (1)

33.1

33.7

5.81

5.73

8

4.5 (0)

5.0 (1)

55.7

56.5

4.58

4.60

9

8.0 (1)

5.0 (1)

63.9

62.6

4.10

4.08

10

4.5 (0)

2.8 (0)

68.4

68.3

5.94

5.80

11

4.5 (0)

2.8 (0)

68.5

68.3

5.94

5.80

12

4.5 (0)

2.8 (0)

68.2

68.3

5.94

5.80


(-1), (0) and (1) are coded levels

Barghini et al. Microbial Cell Factories 2007 6:13   doi:10.1186/1475-2859-6-13

Open Data