Figure 2.

SDS-PAGE analysis of cell extracts after lysis of bacteria and purified proteins. Cell lysates (C) and purified proteins (P) were mixed with 4xSDS-PAGE sample buffer. Protein bands were visualized by Coomassie staining. Yields of purified proteins were classified as indicated with the numbers below the protein bands (3: high, 2: moderate1: low). Numbers on top of the panel designate proteins as given in additional file 1. Note that only one clone per protein was chosen for large-scale purification.