|
Gateway recombination cloning of 34 LUCA and ECHH genes |
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| No. |
Protein |
Yield with His7-tag |
Fusion with |
Yield |
|
|
||||
| 157 |
aspartokinase I; homoserine dehydrogenase I |
1 |
MBP |
3 |
| 145 |
acetolactate synthase III small subunit |
1 |
MBP |
3 |
| 203 |
thiogalactoside acetyltransferase |
1 |
MBP |
2 |
| 200 |
4-hydroxy-2-ketovalerate aldolase |
1 |
MBP |
2 |
| 258 |
putative ATPase |
1 |
MBP |
3 |
| 204 |
anthranilate synthase component II |
1 |
MBP |
2 |
| 155 |
putative ATP-binding component of a transport system |
1 |
MBP |
3 |
| 201 |
flagellum-specific ATP synthase |
1 |
MBP |
2 |
| 259 |
fused enoyl-CoA hydratase and epimerase |
1 |
MBP |
2 |
| 156 |
FFh |
1 |
MBP |
2 |
| 146 |
30S ribosomal subunit protein S11 |
1 |
MBP |
2 |
| 147 |
30S ribosomal subunit protein S13 |
1 |
MBP |
3 |
| 148 |
50S ribosomal subunit protein L14 |
1 |
MBP |
3 |
| 149 |
50S ribosomal subunit protein L22 |
1 |
MBP |
3 |
| 151 |
50S ribosomal subunit protein L23 |
1 |
MBP |
2 |
| 152 |
30S ribosomal subunit protein S12 |
1 |
MBP |
2 |
| 150 |
50S ribosomal subunit protein L2 |
1 |
MBP |
3 |
| 154 |
50S ribosomal subunit protein L1 |
1 |
MBP |
3 |
| 153 |
biotin- [acetyl-CoA carboxylase] holoenzyme synthetase |
1 |
MBP |
3 |
| 159 |
RNA polymerase beta prime subunit |
1 |
MBP |
2 |
| 143 |
predicted acyltransferase with acyl-CoA N-acyltransferase |
1 |
MBP |
3 |
| 144 |
putative proteoglycan |
1 |
MBP |
2 |
| 158 |
endonuclease III |
1 |
MBP |
2 |
| 161 |
dihydroxyacid dehydratase |
1 |
MBP |
3 |
| 202 |
part of formate-dependent nitrite reductase complex |
1 |
NusA |
2 |
| 206 |
conserved protein, member of DEAD box family |
1 |
NusA |
2 |
| 198 |
50S ribosomal subunit protein L5 |
0 |
NusA |
2 |
| 205 |
O-6-alkylguanine-DNA/cysteine-protein methyltransferase |
0 |
NusA |
1 |
| 199 |
acetylornithine delta-aminotransferase |
0 |
NusA |
1 |
| 197 |
inducible ATP-independent RNA helicase |
1 |
GST |
2 |
| 160 |
glutamate synthase large subunit |
1 |
- |
|
| 207 |
carbamoyl-phosphate synthase large subunit |
1 |
- |
|
| 208 |
ATP-binding transport protein (membrane protein) |
0 |
- |
|
| 209 |
Mg2+ transport ATPase, P-type 1 (membrane protein) |
0 |
- |
|
|
Yields of purified proteins were analyzed by SDS-PAGE before recombination (proteins fused to His7-tag) and after recombination (each target protein fused to MBP-His7, GST-His7, or NusA-His6, respectively). The fusion partner that most efficiently improved protein yield is shown. With regard to yield, proteins were attributed to one of four categories: 3 (high), 2 (moderate), 1 (low), 0 (not purified). | ||||
Ergin et al. Microbial Cell Factories 2007 6:18 doi:10.1186/1475-2859-6-18 |
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