Figure 2.

Impact of the cultivation temperature on the scFv production. Production of functional antibodies was analyzed by antigen binding ELISA of D1.3 scFvs of 50 μL culture supernatant after 24 h production in 100 mL scale. Mean values and standard deviations of data obtained from three different cultures are given. Antigens: 1 μg/well lysozyme or 1 μg/well control protein BSA. The D1.3 antibodies were detected using mAb mouse anti-His and goat anti-mouse IgGHRP (Fab specific).