Figure 3.

Impact of different culture media on the production of functional scFvs. A Antigen binding ELISA with 50 μL culture supernatant from 18 h production in 100 mL scale, performed as described in figure 2. Mean values and standard deviations of data obtained from three different cultures are given. B Immunoblot of culture supernatant. Ammonium sulfate precipated scFvs from 1.5 mL supernatant were separated by reducing SDS-PAGE (12 %) and detected using mAb mouse anti-His and goat anti-mouse IgG AP (Fab specific).