Figure 4.

Impact of the production time on the yield of functional scFvs (TB medium, 41°C). A Antigen binding ELISA with 50 μL culture supernatant from different time points, performed as described in figure 2 and 3. Mean values and standard deviations of data obtained from three different cultures are given. B Immunoblot of produced scFvs. Ammonium sulphate precipated scFvs from 1 mL supernatant were separated by reducing SDS-PAGE (12 %) and detected as described in figure 3.