Additional file 1.

Signal peptide prediction. The amino acid sequence of rAra h 2 is submitted to the prediction server http://www.cbs.dtu.dk/services/SignalP/ using the gram positive bacteria as settings. The C-score is the cleavage site score. For each position in the submitted sequence, a C-score is reported, which should only be significantly high at the cleavage site. Y-max is a derivative of the C-score combined with the S-score resulting in a better cleavage site prediction than the raw C-score alone. This is due to the fact that multiple high-peaking C-scores can be found in one sequence, where only one is the true cleavage site. The cleavage site is assigned from the Y-score where the slope of the S-score is steep and a significant C-score is found. The S-mean is the average of the S-score, ranging from the N-terminal amino acid to the amino acid assigned with the highest Y-max score, thus the S-mean score is calculated for the length of the predicted signal peptide.

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Glenting et al. Microbial Cell Factories 2007 6:28   doi:10.1186/1475-2859-6-28