Figure 2.

Western immunoblot demonstrating recognition of six novel P. multocida antigens by pooled sera from experimentally infected chickens. Sizes of markers in kilodaltons are indicated on the left. Lanes: 1, protein molecular standards (kDa) (SeeBlue Plus2 Prestained Sandard, Novagen); 2 and 9 are blank; 3, PM0442 (pLIC-Nus); 4, PM0979 (pDEST-17); 5, PM0659 (pDEST-17); 6, PM1993 (pDEST-17); 7, PM1614 (pLIC-Nus); 8, PM1979 (pLIC-Nus); 10, P. multocida X73 whole cell lysate. All the soluble fusion proteins cloned with a NusA tag were cleaved and detected after incubation with Tev protease at room temperature for 2 h. The top arrows in the corresponding lanes show the expected size of the fusion proteins and the bottom arrows mark the recombinant proteins without the NusA tag. Lanes with single arrows correspond to the recombinant proteins expressed in the absence of a NusA tag.