Figure 5.

DAHP production by strain PB12 PTS^-Glc⁺(pRW300aroG^fbr pCLtktA). Fermentor cultures of strain PB12 carrying these plasmids, grown aerobically in minimal medium, containing either a final concentration of 4 g/L equivalent to approximately 130 mmolC/L (in the case of one carbon source), or 2 g/L equivalent to approximately 65 mmolC/L, of each carbohydrate (when mixtures of two were utilized). Acetate concentrations were also measured in these fermentations and are reported in table 4. Mean values from two independent cultures are shown. Differences between values in these experiments were <10%. IPTG (0.1 mM) was included in the medium at the beginning of the fermentations.