Microbial Cell Factories
Volume 7
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 ReviewFolding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometryBohumila Suchanova1  and Roman Tuma1,2  1Institute of Biotechnology, University of Helsinki, Finland 2Astbury Centre for Structural Molecular Biology, University of Leeds, UK  author email corresponding author email
Microbial Cell Factories 2008,
7:12doi:10.1186/1475-2859-7-12 Abstract
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community. | 
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