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Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae

Laura Salusjärvi1 email, Matti Kankainen2 email, Rabah Soliymani3 email, Juha-Pekka Pitkänen1 email, Merja Penttilä1 email and Laura Ruohonen1 email

VTT, Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland

Structural Genomics, Institute of Biotechnology, P.O. Box 56, University of Helsinki, FI-00014 HY, Finland

Protein Chemistry Unit, Institute of Biomedicine, Anatomy Biomedicum-Helsinki, P.O. Box 63, University of Helsinki, FI-00014 HY, Finland

author email corresponding author email

Microbial Cell Factories 2008, 7:18doi:10.1186/1475-2859-7-18

Published: 4 June 2008

Additional files

Additional file 1:

Pearson correlation coefficient values between the biological and technical replicate arrays from samples of glucose fermentations. The data provided shows the Pearson correlation coefficient values between the biological and technical replicate arrays of from samples of glucose fermentations (Glc5h and Glc24h).

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Additional file 2:

Pearson correlation coefficient values between the biological and technical replicate arrays of samples from xylose fermentations. The data provided shows the Pearson correlation coefficient values between the biological and technical replicate arrays of xylose samples from fermentations (Xyl72h).

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Additional file 3:

Scatterplots of RMA pre-processed arrays from cells grown on glucose for 5 h. The figure provided represents the scatterplots of the expression values of the replicate microarrays hybridised with the samples derived from cells grown on glucose for 5 h.

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Additional file 4:

Scatterplots of RMA pre-processed arrays from cells grown on glucose for 24 h. The figure provided represents the scatterplots of the expression values of the replicate microarrays hybridised with the samples derived from cells grown on glucose for 24 h.

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Open Data

Additional file 5:

Scatterplots of RMA pre-processed arrays from cells grown on xylose for 72 h. The figure provided represents the scatterplots of the expression values of the replicate microarrays hybridised with the samples derived from cells grown on xylose for 72 h.

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Open Data

Additional file 6:

Cluster 1. List of open reading frames in cluster 1 shown in Fig. 2 of the paper.

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Additional file 7:

Cluster 2. List of open reading frames in cluster 2 shown in Fig. 2 of the paper.

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Additional file 8:

Cluster 3. List of open reading frames in cluster 3 shown in Fig. 2 of the paper.

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Additional file 9:

Cluster 4. List of open reading frames of in cluster 4 shown in Fig. 2 of the paper.

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Additional file 10:

Cluster 5. List of open reading frames in cluster 5 shown in Fig. 2 of the paper.

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Additional file 11:

Cluster 6. List of open reading frames of in cluster 6 shown in Fig. 2 of the paper.

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Additional file 12:

Cluster 7. List of open reading frames in cluster 7 shown in Fig. 2 of the paper.

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Open Data

Additional file 13:

Cluster 8. List of open reading frames in cluster 8 shown in Fig. 2 of the paper.

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Additional file 14:

Image of the 11% SDS-PAGE 2-DE-gel. The image of the 2-DE-gel showing the locations of the seventy protein spots, which had different abundance in cells growing on glucose or xylose.

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Additional file 15:

Clustering of the proteome data. The figure shows seventy proteins, which were differentially translated in the glucose repressed, glucose derepressed and xylose-grown cells and clustered by using hierarchical clustering with Euclidean distance and average linkage.

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Additional file 16:

Expression profiles of genes involved in cyclic AMP – phosphokinase A pathway (cAMP-PKA) of S. cerevisiae. The figure provided shows the expression trend of genes involved in cyclic AMP – phosphokinase A pathway in cells grown on glucose for 5 or 24 h or on xylose for 72 h.

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Additional file 17:

Images of 2-DE gels with phosphorylated proteins. Images of 2-DE gels showing the locations of Hxk2p, Glk1p, Eno2p and Eno1p in samples from cells grown for 72 h on xylose and for 5 h on glucose and stained either with phosphoprotein specific Pro-Q Diamond or Sypro Ruby.

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