Table 3

Riboflavin accumulation of P. pastoris strains, overexpressing two RIB genes

Strain

Riboflavin in supernatant

X-33 ScRIB1

3.9 mg/L +/- 0.25

GS115 PpRIB1

3.0 mg/L +/- 0.10

GS115 PpRIB1+empty

3.0 mg/L +/- 0.18

GS115 PpRIB1+1

7.1 mg/L +/- 0.04

GS115 PpRIB1+3

10.1 mg/L +/- 0.24

GS115 PpRIB1+7

4.9 mg/L +/- 0.19

The cells were cultivated in shake flasks on defined medium and glucose as carbon source. Shake flask experiments and HPLC measurements were run in duplicates. Mean values +/- standard deviations are given.

Marx et al. Microbial Cell Factories 2008 7:23   doi:10.1186/1475-2859-7-23