Figure 2.

Illustrative partial DNA and amino acid sequence of the constructed vector and LLO respectively. (A) Illustrative DNA sequence of the SOE (splicing by overlap extension) PCR product consisting of the PnisA promoter spliced to downstream six-His-tagged hly gene of L. monocytogenes EGD-e. Flanking restriction sites of BglII and KpnI exist on the PnisA and hly sides respectively. The six-His-tag codons are underlined. (B) Comparison between the amino acid sequence of native LLO (L. monocytogenes EGD-e) with the signal sequence and the constructed six-His-tagged LLO produced in this study.