Table 1

Table 1
Bacterial strains and plasmid vectors used in the present study
Strain or plasmid name	Description	Reference or source

E. coli Top10	Chemically-competent intermediate host, plasmid free	Invitrogen
E. coli BL21	Chemically-competent E. coli, used in this study as an intermediate host for pQE30, plasmid free	Novagen
Listeria monocytogenes EGD-e serovar 1/2a	Wild type Listeria monocytogenes	[32]
Lactococcus lactis NZ9700	Nisin producer strain	[13]
Lactococcus lactis NZ9000	L. lactis subsp. Cremoris MG1363 carrying nisRK on the chromosome	[13]
L. lactis NZ9000 (pNZPnisA:CYTO-LLO)	Lactococcus lactis NZ9000 harbouring pNZPnisA:CYTO-LLO plasmid and over-expressing LLO upon nisin induction	This study
pQE30	Expression vector using phage T5 promoter and adding an N-terminus six-His tag to the expressed protein, Amp^R(ampicillin resistant)	Qiagen
pQE30/hly	pQE30 vector with hly gene (without signal sequence) inserted between BamHI and PstI restriction sites.	This study
pNZ8048	E. coli-L. lactis shuttle vector containing PnisA promoter and start codon in NcoI site, Cm^R(chloramphenicol resistant)	[13]
pNZPnisA:CYTO-LLO	Modified pNZ8048 containing PnisA promoter (NcoI site eliminated) with downstream His-tagged hly gene, Cm^R	This study

Bahey-El-Din et al. Microbial Cell Factories 2008 7:24 doi:10.1186/1475-2859-7-24