Table 2 |
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|
Oligonucleotide primers used in this study. |
||
| Primer code number |
Primer name |
Primer sequence (5'-3') (restriction enzyme site or overhang)a |
|
|
||
| 1 |
pQE30 forward primer |
GAAGGATCCGATGCATCTGCATTCAATAAAG (BamHI site) |
| 2 |
pQE30 reverse primer |
ACGCCTGCAGTTCGATTGGATTATCTACTTTATTA (PstI site) |
| 3 |
PnisA forward primer |
CCAAGATCTAGTCTTATAACTATACTG (BglII site) |
| 4 |
PnisA reverse primer (hly overhang) |
GGTGATGTCCCATTTTGAGTGCCTCCTTATAATTTATTTTG (hly overhang) |
| 5 |
hly forward primer (PnisA overhang) |
AGGCACTCAAAATGGGACATCACCATCACCATCACGGA (PnisA overhang) |
| 6 |
hly reverse primer |
AGTCGGTACCTTATTCGATTGGATTATCTAC (KpnI site) |
|
(a) Restriction enzyme recognition sites are italicized and underlined while SOE overhangs are underlined. | ||
Bahey-El-Din et al. Microbial Cell Factories 2008 7:24 doi:10.1186/1475-2859-7-24 |
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