|
Comparative results of LLO production and purification by different investigators. |
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| Source of LLO |
Starter Culture volume (litre) |
LLO yield (mg l-1) {Total yield (mg)} |
Specific LLO haemolytic activity (HU per mg protein) pH 5.5 |
Expression host |
Plasmid expression system/inducer (If applicable) |
Reference |
|
|
||||||
| Culture supernatant |
27 |
0.022 {0.6} |
106 (pH 6) |
Listeria monocytogenesa |
N/A |
[24] |
| Culture supernatant |
3 |
1.6 {4.8} |
1.02 × 106 (pH 5.7) |
Listeria innocuab |
pERL3-503 (constitutive) |
[26] |
| Culture supernatant |
6 |
0.25 {1.5} |
2.6 × 105 |
LLO-hypersecretor Listeria monocytogenesa |
N/A |
[25] |
| Cell lysate |
1 |
4.5 {4.5} |
1.25 × 106 |
Escherichia colic |
pET system/IPTG |
[15] |
| Cell lysate |
0.6 |
3.5 – 8 {2.1 – 4.8} |
1.8 × 106 |
Escherichia colid |
pQE31 system/IPTG |
[14] |
| Cell lysate |
0.6 |
2.5 {1.5} |
2.16 × 106 |
Escherichia colie |
pQE70 system/IPTG |
[14] |
| Cell lysate |
0.5 |
-Single sonication treatment: 4.43 – 5.9 {2.215 – 2.95} -Double sonication approach: 9.3 – 12.9 {4.65 – 6.45} |
5 × 105 – 5 × 107 |
Lactococcus lactis NZ9000d |
NICE system (pNZPnisA:CYTO-LLO)/nisin |
This study |
|
(N/A) not applicable; IPTG: Isopropyl β-D-1-thiogalactopyranoside; a untagged native full-length LLO; b untagged full-length LLO; c untagged LLO without signal sequence; d N-terminus His-tagged LLO without signal sequence; e C-terminus His-tagged LLO without signal sequence | ||||||
Bahey-El-Din et al. Microbial Cell Factories 2008 7:24 doi:10.1186/1475-2859-7-24 |
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