Heme and menaquinone induced electron transport in lactic acid bacteria

doi:10.1186/1475-2859-8-28

Microbial Cell Factories

Volume 8

Viewing options:

Abstract
Full text
PDF (721KB)
Additional files

Associated material:

Related literature:

Articles citing this article
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Brooijmans R
Smit B
Santos F
van Riel J
de Vos WM
Hugenholtz J

on PubMed

Brooijmans R
Smit B
Santos F
van Riel J
de Vos WM
Hugenholtz J
Related articles/pages
on Google

on Google Scholar

on PubMed

Tools:

Post to:

Research

Heme and menaquinone induced electron transport in lactic acid bacteria

Rob Brooijmans¹^,2 , Bart Smit³ , Filipe Santos¹^,2 , Jan van Riel⁴ , Willem M de Vos² and Jeroen Hugenholtz¹^,4

¹TI food & Nutrition, Kluyver Centre for Genomics of Industrial Fermentation, Po Box 557, 6700 AN, Wageningen, the Netherlands

²Wageningen University and Research Centre, Laboratory of Microbiology, Dreijenplein 10, Building 316, 6703 HB, Wageningen, the Netherlands

³Campina Innovation, Nieuwe Kanaal 7C, 6709PA, Wageningen, the Netherlands

⁴NIZO food research, PO Box 20, 6710 BA Ede, the Netherlands

author email corresponding author email

Microbial Cell Factories 2009, 8:28doi:10.1186/1475-2859-8-28


Published:	29 May 2009

Abstract

Background

For some lactic acid bacteria higher biomass production as a result of aerobic respiration has been reported upon supplementation with heme and menaquinone. In this report, we have studied a large number of species among lactic acid bacteria for the existence of this trait.

Results

Heme- (and menaquinone) stimulated aerobic growth was observed for several species and genera of lactic acid bacteria. These include Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacilllus brevis, Lactobacillus paralimentarius, Streptococcus entericus and Lactococcus garviae. The increased biomass production without further acidification, which are respiration associated traits, are suitable for high-throughput screening as demonstrated by the screening of 8000 Lactococcus lactis insertion mutants. Respiration-negative insertion-mutants were found with noxA, bd-type cytochrome and menaquinol biosynthesis gene-disruptions. Phenotypic screening and in silico genome analysis suggest that respiration can be considered characteristic for certain species.

Conclusion

We propose that the cyd-genes were present in the common ancestor of lactic acid bacteria, and that multiple gene-loss events best explains the observed distribution of these genes among the species.