Components of the E. coli envelope are affected by and can react to protein over-production in the cytoplasm

Additional file 1:

On-plate detection of lipase activity. LB-agar plate containing 0.1 mM IPTG and 1% (v/v) tributyrin. Strains were grown overnight at 37°C then incubated at 30°C for 24 h. The presence of lipase activity is indicated by a clear hydrolysis halos around cells. A BL21 strain bearing an active lipase (PFL, [30]) was also plated as a positive control.

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Additional file 2:

Pre-induction is required for SDS-dependent growth impairment. Spots plated on IPTG-deprived (left) and IPTG-containing (middle) control Petri dishes or in the presence of SDS but without IPTG (right). First three columns of each plate are derived from un-induced fermentations; the last 3 ones from 0.1 mM IPTG induced cultures. Spot range between pure cultures to 5-fold dilutions thereof by means of 1:10 steps. The accumulation of active GFP causes cells to assume a greenish yellow colour. Used abbreviations: C+, control; G, GFP; B, BCL.

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Additional file 3:

Set of gels used for proteomic analysis. 2DE-gel pairs in the pI and MW range of interest. Arrows indicate over-expressed proteins.

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