SDS-PAGE analysis of samples during TreS purification. All the protein samples were loaded onto a 12.5% polyacrylamide gel under denaturing conditions. The gel was stained with Coomassie Brilliant Blue R-250. The arrow indicates recombinant TreS. Lane 1, control: Cell lysate of E. coli BL21 (DE3) plysS with pET30a(+); lane 2, cell lysate before induction; lane 3, cell lysate after induction; lane 4, flow through of the Ni-NTA column; lane 5, wash by 20 mM imidazole; lane 6, wash by 40 mM imidazole; lane 7, wash by 60 mM imidazole; lane 8, purified TreS; lane M, molecular weight marker (Bomaide, Beijing, China).
Yue et al. Microbial Cell Factories 2009 8:34 doi:10.1186/1475-2859-8-34