IC assay of reaction products. Reaction mixtures containing 400 μl purified TreS solution and 100 μl maltose (500 mM) substrate in 50 mM potassium phosphate buffer (pH 6) were incubated at 37°C for 2 h. The reaction mixtures were subsequently analyzed by IC, as described in the "Methods" section. Peak 1, trehalose; peak 2, glucose; peak 3, buffer reagent; peak 4, maltose.
Yue et al. Microbial Cell Factories 2009 8:34 doi:10.1186/1475-2859-8-34