Table 2

Effects of metal ions and chemicals on the activity of recombinant TreS

Reagent

Relative activity (%)

Reagent

Relative activity (%)


None

100*

Cu2+

52 ± 4.7

Hg2+

57 ± 2.2

Na+

90 ± 2.4

Mg2+

98 ± 1.3

NH4 +

101 ± 4.3

Ca2+

103 ± 7.9

SDS

10 ± 1.1

Zn2+

59 ± 1.3

EDTA

98 ± 5.8

Fe2+

92 ± 5.9

β-ME

0

Mn2+

111 ± 10.7


* The enzyme activity of TreS without the addition of ions or reagents was taken to be 100%.

Yue et al. Microbial Cell Factories 2009 8:34   doi:10.1186/1475-2859-8-34

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