Table 1

Cell culture performance parameters

Bioreactor Comparison 1

Bioreactor Comparison 2


Parameter

CultiBag

HTBR

CultiBag

HTBR


culture volume

2 L

35 ml

3.5 L

35 ml

peak vcd (× 106 cells/ml)

1.28

1.18 ± 0.24

0.76

0.70 ± 0.18

final IVCD (× 106 cells/ml-h)

59.70

50.63 ± 9.89

38.39

23.85 ± 8.05

peak mAb concentration (mg/l)

92.92

92.16 ± 7.08

42.41

26.65 ± 4.51

average growth rate (1/h)

0.026

0.029 ± 0.007

0.036

0.022 ± 0.005

average mAb productivity (pg/cell-d)

37.35

44.87 ± 12.08

32

28.64 ± 5.70


Summary of growth and mAb production during the bioreactor comparisons. All reactors from the same comparison inoculated from the same pool of cells. N = 4 and 3 for HTBR bioreactors in Bioreactor Comparisons 1 and 2, respectively.

Hanson et al. Microbial Cell Factories 2009 8:44   doi:10.1186/1475-2859-8-44

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