Table 1 |
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|
Cell culture performance parameters |
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|
Bioreactor Comparison 1 |
Bioreactor Comparison 2 |
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|
|
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|
Parameter |
CultiBag |
HTBR |
CultiBag |
HTBR |
|
|
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|
culture volume |
2 L |
35 ml |
3.5 L |
35 ml |
|
peak vcd (× 106 cells/ml) |
1.28 |
1.18 ± 0.24 |
0.76 |
0.70 ± 0.18 |
|
final IVCD (× 106 cells/ml-h) |
59.70 |
50.63 ± 9.89 |
38.39 |
23.85 ± 8.05 |
|
peak mAb concentration (mg/l) |
92.92 |
92.16 ± 7.08 |
42.41 |
26.65 ± 4.51 |
|
average growth rate (1/h) |
0.026 |
0.029 ± 0.007 |
0.036 |
0.022 ± 0.005 |
|
average mAb productivity (pg/cell-d) |
37.35 |
44.87 ± 12.08 |
32 |
28.64 ± 5.70 |
|
|
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|
Summary of growth and mAb production during the bioreactor comparisons. All reactors from the same comparison inoculated from the same pool of cells. N = 4 and 3 for HTBR bioreactors in Bioreactor Comparisons 1 and 2, respectively. |
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|
Hanson et al. Microbial Cell Factories 2009 8:44 doi:10.1186/1475-2859-8-44 |
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